In EZ-Agarose (Model L), EB dye is stored in the anode buffer reservoir. It is separated from
the gel matrix by a buffer dam. The buffer dam needs to be opened to let the
anode buffer connect to the gel for electrophoresis. A special tool is provided to
make this action effortlessly.
The buffer dam has been opened (Model L). There is no buffer dam for Model R.
See User's Manual for Detail