Instant-Bands
View Your Protein Bands in an SDS Gel instantly |
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Directly view protein bands while the gel is still in its cassette |
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Instant-Bands Sample Treatment Buffer (Sample Loading Buffer) stains protein samples for SDS-PAGE. Protein samples are pre-stained during sample treatment prior to electrophoresis. The protein bands in a gel can be visualized and pictured directly after electrophoresis by a gel image system or by a simple UV or LED transilluminator. This product has the following features:
Simple: Completely eliminate the staining/de-staining procedure without introducing any extra steps to the standard SDS-PAGE protocol
Fast: The results can be visualized and analyzed instantly after electrophoresis while the gel is still in its cassette
Sensitive: The sensitivity is higher than the silver staining
Flexible: Gels pre-stained with Instant-Bands can be transferred for Western Blot experiment. It can also be further stained by Coomassie blue if desired
Clean: No contamination of organic solvents to our environment and no messes of coomassie blue stains in your lab
| Click Order button at the top of this page to try Instant-Bands Testing Sample ($24) |
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Instant ResultInstant-Band treated samples are directly viewed on an UV transilluminator (1) and the same gel is then stained by Coomassie blue (2). Lane 2, 4, 6, 8 and 10 were treated by Instant-Bands loading buffer. Lane 1, 3, 5, 7 and 9 were treated by the regular loading buffer. |
| You don’t need to retrieve the gel from the gel cassette In this image, the gel was viewed by a LED transilluminator while the gel was still in the gel cassette. The protein bands can also be viewed by any other gel image systems. LED transilluminator is now widely available in many labs. It is initially developed for Agarose gel stained by safe DNA dyes. The excitation wavelength of Instant-Band is within visible range that perfectly matches light emitted by a blue LED light. So LED transilluminator is ideally suitable to Instant-Bands stained protein SDS gel. |
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Compatible with Western Blot ExperimentPonceau S stain is no more needed After transferred to membrane, Instant-Bands stained protein bands become more intense because a membrane is much thinner than a gel. Protein samples stained by Instant-Bands can then be directly detected by Western Blot. Alternatively, you can load an Instant-Bands treated sample next to the well of the same sample treated by the regular loading buffer. The Instant-Bands sample serves as the control for transfer efficiency and for location of the target protein. |
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Using Instant-Bands to eliminate gel stain and de-stain, the entire process from expression to purification is streamlined without interruption. It greatly accelerates and simplifies this .
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| Gel 1: Expression and solubility of the target protein The picture was directly taken while the gel was still in its cassette. It showed that the target protein was expressed and was soluble. So the experiment was immediately moved to the purification step. Lane 1, fluorescent protein M.W makers Lane 2, cells without induction Lane 3, cells induced by IPTG Lane 4, supernatant of cell lysis Lane 5, pellet of cell lysis |
Gel 2: Purification of the expressed protein Again, the gel was still in the cassette Lane 1, fluorescent protein M.W makers Lane 2, supernatant of cell lysis before Ni column Lane 3, Ni column flow through Lane 4, wash with 10 mM imidazole Lane 5, wash with 30 mM imidazole Lane 6, wash with 100 mM imidazole Lane 7, elution with 400 mM imidazole Lane 8, elution with 1 M imidazole |
| Related Products |
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Instant-Bands is a 3x sample treatment buffer. It comes in various sizes. Each package contains 3 vials: Instant-Bands, Resuspension Buffer and Enhancing buffer. Enhancing Buffer is only needed for Bis-tris gel. Functionally, there are the reduced Instant-Band and the non-reduced Instant-Band, which are for reducing gel (the most common gel) and non reducing gel, respectively. |
| Prestaining Protein Sample Treatment Buffer | Catalog # | Price | |
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Instant-Bands, for SDS-PAGE, Double size package2 vials. Re-suspend each vial in 1000 ul resuspension buffer before use. |
PFS001P |
$135.00 |
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Instant-Bands, for SDS-PAGE, Regular size package1 vial. Re-suspend each vial in 1000 ul water before use. |
PFS001-RP |
$85.00 |
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Instant-Bands, for SDS-PAGE, Testing Sample package1 vial. Re-suspend each vial in 100 ul resuspension buffer before use. |
PFS001-TP |
$24.00 |
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Instant-Bands, for non-reducing gel, Double size package2 vials. Re-suspend each vial in 1000 ul resuspension buffer before use. |
PFS002P |
$135.00 |
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Instant-Bands, for non-reducing gel, Regular size package1 vial. Re-suspend each vial in 1000 ul resuspension buffer before use. |
PFS002-RP |
$85.00 |
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Instant-Bands, for non-reducing gel, Testing Sample package1 vial.Re-suspend each vial in 100 ul resuspension buffer before use. |
PFS002-TP |
$24.00 |
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| Product | Catalog # | Price | |
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MKP004 |
$125.00 |
| Product | Catalog # | Price | |
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Instant-Bands + Fluorescent Protein M.W. MarkersPFS001+MKP004 |
PSFMKP |
$208.00 |
