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Polyclonal Antibody: Protocol

Standard Protocol
  week 0 pre-immu serum collection Pre-bleed (~ 2 ml)
  week 0 Primary injection 0.5 - 1.0 mg peptide-KLH emulsified with CFA injected subcutaneously
~ week 2 1st boost 0.5 - 1.0 mg peptide-KLH emulsified with IFA injected subcutaneously
~ week 4 2nd boost 0.5 - 1.0 mg peptide-KLH emulsified with IFA injected subcutaneously
~ week 6 Serum collection Bleed 1 ml per rabbit and Elisa to check titer
~ week 7 3rd boost 0.5 - 1.0 mg peptide-KLH emulsified with IFA injected subcutaneously
~ week 9 Serum Collection Bleed 1 ml per rabbit
~ week 10 ELISA, Serum collection and shipping Verify and terminate
Extended Protocol    ( continue from week 9 )
~ week 10 ELISA and serum collection 1 ~10 ml per rabbit
~ week 11 antigen injection  
~ week 12 serum collection 2 ~10 ml per rabbit
~ week 13 antigen injection  
~ week 14 serum collection 3 ~10 ml per rabbit
Note for extented protocol:
The protocol will be extended according to customers' request. For serum collection more than 4 times ( including final termination),
extra peptide synthesis and conjugation are required.
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